Fluorescence is a common problem in Raman spectroscopy and longer excitation wavelengths is the answer. Under conditions where fluorescence is generated, it may be intense and can overshadow the Raman features. Fluorescence emission stems from sample molecules or trace impurities that absorb the laser excitation and emit a broad background at the same energies as the Raman scattering. One way to eliminate or reduce the fluorescence emission is to select a laser excitation wavelength that does not have enough energy to excite molecular fluorescence.

The Advantage 785™ reduces competing fluorescence interference in compounds through this process. It uses a 785 nm excitation laser to reduce the fluorescence signature in samples that show strong fluorescence at shorter wavelengths. We also offer a microscope attachment, the NuScopeTM, which is used to image samples and show Raman spectra of domains in heterogeneous materials.